Autosampler Settings

The rate sample injection and Maxpar Cell Acquisition Solution Plus (CAS Plus) flow from the Autosampler to the nebulizer. The default flow rate, and necessary flow rate for instrument tuning and data acquisition, is 30 μL/min. This setting can be changed by a CyTOF Administrator, only for troubleshooting purposes.

IMPORTANT  A change to flow rate made by a CyTOF Administrator affects all accounts. CyTOF Users cannot change the flow rate. Be sure to set the flow rate back to 30 μL/min before resuming tuning or data acquisition activities.

The maximum number of clogs that can occur within an hour before acquisition is stopped.

The pressure at which the automatic unclogging routine is initiated. The default value is 15 psi.

The pressure at which the automatic unclogging routine exits and acquisition resumes.

The acquisition delay applied after injection of the first sample loop starts. This delay allows time for the sample to load and travel from the sample loop to the nebulizer.

The acquisition delay applied between loops to account for the delay between the end of the previous loop and the start of the next.

The time required will be less than the First Loop Delay Time.

The default flow rate of liquid from the Autosampler to the nebulizer during wash cycles. The default Wash Flow Rate is 50 µL/min. This setting is independent of the acquisition flow rate.

CAS Plus rinse time that precedes Medium and Heavy wash cycles to rinse the nebulizer during manually initiated wash cycles. Equivalent to a Light wash cycle, which is CAS Plus rinse only.

The Medium wash duration in minutes. Washing solution followed by CAS Plus rinse.

The Heavy wash duration in minutes. Washing solution with pulsing to clear the nebulizer, followed by CAS Plus rinse.

The volume of Maxpar Water for CyTOF XT (PN 201242) used for the high speed loop flush. For Medium and Heavy washes, the system rinses the loop after the acid wash is finished. This is used for all 3 washes.

The location of the sample in the carousel.

The sample volume in µL.

NOTE   The sample volume is injected in 250 µL aliquots (the sample loop volume). The volume of the final loop includes an additional 12 µL to account for the Autosampler Nebulizer Line (PN 117784), so the total injection volume is Volume + 12 µL.

When selected, EQ™ Six Element Calibration Beads (or EQ Four Element Calibration Beads for samples prepared with Maxpar® Direct™ Immune Profiling Assay™), at 10% of the total sample volume, are added to the sample by the Autosampler.

Check to agitate the sample loop for 400 ms every 2 sec during injection and washing. This setting is recommended to prevent cells from settling in the sample loop during acquisition and to reduce the risk of clogs.

Choose Suspension for cell samples that contain suspension solution and require mixing. Choose Pellet for cell pellet samples that require resuspension.

Check to have the remaining sample returned to the tube after acquisition is complete or interrupted.

NOTE   Approximately 30 µL of CAS Plus is returned to the tube with the sample.

The number of times the probe aspirates and expels the sample during mixing.

The relative velocity of the sample when dispensed into the tube during mixing. The default setting is 10.